Antibacterial and Antioxidant Activity of Isoflavans from the Roots of Rhynchosia ferruginea and In Silico Study on DNA Gyrase and Human Peroxiredoxin

Rhynchosia ferruginea (Udusalim, Afan Oromo) is a medicinal plant traditionally used to treat skin infection, intestinal problems and amoebiasis. Silica gel chromatographic separation of dichloromethane/methanol (1:1) roots extract yielded isoflavan (1), isoflavene (2) 1, 3-dilinoleoyl-2-stearoylglycerol (3), reported herein for the first time from genus. Antibacterial activity was examined using disc diffusion method against E. coli, S. aureus, P. aeruginosa pyogenes. AutoDoc vina 4.2 soft ware molecular docking analysis compounds human peroxidoxin 5 DNA gyrase B enzymes. Isoflavan (1) displayed zone inhibition 9.67 ± 0.58 mm 10.67 whereas showed 10.33 1.15 10 1.00 coli respectively, compared ciprofloxacin (15.67±0.58 both strains). DPPH radical scavenging assay exhibited better with IC50 value 17.7 32, respectively. Molecular revealed that 1 2 similar binding affinity -7.4 kcal/mol (-7.3 kcal/mol). In silico (PDB ID: 1HD2) minimum - 3.7 2.0 2, ascorbic acid (-4.9 The in vitro antibacterial antioxidant suggest potential use these as drug lead candidates which corroborate traditional uses R. ferruginea.